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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: J Steroid Biochem Mol Biol. 2018 Nov 30;187:166–173. doi: 10.1016/j.jsbmb.2018.11.015

Figure 1. Effects of sequential treatment with AraC and D2/CA on the growth and viability of HL60 cells.

Figure 1.

(A, B) Cell death and the total cell number at cell seeding (Untreated-0 h) and following incubation for 72 h with either vehicle (Untreated-72 h) or 100 nM AraC (AraC-72 h), as described in Material and Methods. (C, D) Cell death and the total cell number at seeding of the cells collected after 72 h, as in A and B, (Untreated-0 h; AraC-0 h) and following an additional incubation for 96 h of untreated control (Untreated-96 h) cells or AraC-treated (AraC-96 h) cells, with or without 100 nM 1-D2 and 10 μM CA (D2/CA), as indicated by the rectangles in the Figure. *, p < 0.05; ***, p < 0.001; ****, p < 0.0001 vs. Untreated-72 h or −96 h. ♦, p < 0.05; ♦♦♦♦, p < 0.0001 vs. D2/CA alone; §§§, p < 0.001 vs. Untreated-0 h; #, p < 0.05; ##, p < 0.01; ###, p < 0.001, significant differences between the indicated groups.