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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: J Steroid Biochem Mol Biol. 2018 Nov 30;187:166–173. doi: 10.1016/j.jsbmb.2018.11.015

Figure 3. Knock down of TXNIP by CRISPR/Cas9 in HL60 cells reduces ECD.

Figure 3.

A. Depletion of TXNIP by CRISPR/Cas9 in HL60. The pretreatment with AraC for 72 h, then addition of D2/CA for 96 h. The percentages of cell death, as determined by trypan blue exclusion or annexin V/PI staining, are shown in the table. B. Western blotting to detect the TXNIP downstream targets, ASK1, MKK4 and JNK, as well as the apoptosis-related proteins, BIM and caspase 3. CRK-L was used as a loading control. The average of relative signal intensities from three separate experiments are shown in bar charts above each blot. *, p<0.05; **, p < 0.01 vs the AraC group; #, p<0.01 when comparing CTL CRISPR-ECD with VDR CRISPR-ECD.