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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: J Steroid Biochem Mol Biol. 2018 Nov 30;187:166–173. doi: 10.1016/j.jsbmb.2018.11.015

Figure 4.

Figure 4.

In vitro assays of ASK1 kinase activity showing increased enzyme activity in ECD using two different substrates (note the fourth lane in A and B) to demonstrate consistency. HL60 cell lysates (100 μg) were immunoprecipitated using ASK1 cross-linked column, then incubated with 1 μg of recombinant human p38 or MEK1 proteins as substrates for ASK1-immunoprecipitated kinase assay in the presence of ATP. Western blots were run and probed for Phospho-p38 (Thr180/Tyr182) or for Total-p38, and Phospho-MEK1 (Ser217/221) or Total-MEK1.