Figure 4.

Effect of Rg1 on mitochondrial depolarization. Cells (5 × 10⁶) were treated with Rg1 (0, 50, 70, and 90 μg/mL) for 24 h first and then incubated in media with (model) or without (control) H₂O₂ (100 μmol/L) for an additional 4 h. After that, cells (1 × 10⁵) were incubated with JC-1 (5 μg/mL) and assayed by FCM. Mitochondrial depolarization was presented by a reduction in the red/green fluorescence intensity ratio: (a) control group; (b) model group; (c) 50 μg/mL Rg1 group; (d) 70 μg/mL Rg1 group; (e) 90 μg/mL Rg1 group; (f) bar diagram representing JC-1 polymer/monomer MFI. All data are presented as mean ± S.E. (n = 6). Bars with different letters were significantly different (P < 0.01).