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. 2019 May 6;19:25. doi: 10.1186/s12896-019-0517-7

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Chromatogram showing potential off-target activity on RBFOX1 and AR gene from Ig Heavy chain knockout pig. Similar approach was used to identify any off-target events on RAG2/IL2RG double knockout pigs or SCD5 knockout fetuses. a WT sequence of RBFOX1. b Polymorphic reading from Ig Heavy chain knockout pig E. c Allele specific reading from Ig Heavy chain knockout pig E. d WT sequence of AR. e Polymorphic reading from Ig Heavy chain knockout pig E. f Allele specific reading from Ig Heavy chain knockout pig E. Yellow highlighted portion of sequence indicates deviation from WT sequence reading