Table 3.

Summary of genotypes in Ig Heavy chain gene, AR gene and RBFOX1 gene in embryos injected with 2.5 ng/μL sgRNA and 5 ng/μL Cas9 mRNA. Seven embryos were analyzed for off-targeting events. Heterozygous: WT and one modified allele; Biallelic: two differently modified alleles; Homozygous: one type of modified alleles; Mosaic: more than two types of alleles and the WT sequence may or may not be present

Embryo #	Ig Heavy Chain	AR	RBFOX1
1	Biallelic	Heterozygous	WT
2	Heterozygous	Heterozygous	Heterozygous
3	Homozygous	Mosaic w/o WT	WT
4	Homozygous	WT	Mosaic w/ WT
5	Biallelic	Heterozygous	Heterozygous
6	Biallelic	Heterozygous	Heterozygous
7	Mosaic w/o WT	Heterozygous	Mosaic w/ WT