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. 2019 May 6;12:109. doi: 10.1186/s13068-019-1451-6

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 8 — Quantification of substitution frequency of miscanthus stem and leaf GAX. All characterised GH10 and GH11 hydrolysis products were analysed by DASH using the DASHboard software for quantification. The substitution frequency was calculated after normalisation of values by comparing the abundance side chain to the Xylp residues in the backbone. Error bars represent mean ± SD (n = 3). A significant difference was observed in the D^2,3 substitution frequency of the GH10 digest (p = 0.002) and of the GH11 digest (p = 0.2) between stems and leaves and between the GH10 and G11 digest in leaves only (p = 0.01) using the paired t-test for two-tailed distribution, marked with asterisk