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. 2019 May 6;19:183. doi: 10.1186/s12870-019-1785-z

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — FISH validation of the SNP genotyping of wheat-T. timopheevii introgression lines. Oligos pSc119.2–1 (green) and the Afa family (red) were used as FISH probes. a known FISH karyotype of wheat [56] (b) FISH karyotype of T. timopheevii accession P95–99.1-1 (based on that published by [37]) (c) FISH karyotype of line BC₃F₂–114-1 showing a homozygous introgression from T. timopheevii linkage group 5G into wheat chromosome 5B (d) FISH signals in the metaphase spread of line BC₃F₂–114-1 used to make the karyotype with white arrows indicating chromosomes T5GS.5GL-5BL (e) GGT bar diagram of line BC₃F₂–114-1 showing the T. timopheevii introgression from linkage group 5G in red markers while the wheat alleles are represented in blue