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. 2019 May 6;10:19. doi: 10.1186/s13100-019-0161-8

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Sense and antisense expression profiles of pig L1D of L1 s, SINEA of SINEs, and ERV6B of ERVs. a Primer design for reverse transcription (RT) and real-time quantitative PCR (RT-qPCR) detection. The primer for sense and antisense RT are indicated by red and green arrowheads, respectively, and the primers of ORF1-F/R, ORF2-F/R, 5′UTR-F/R, pol-F/R, gag-F/R, env-F/R, LTR-F/R, SINE-F/R (black arrowheads), are used for RT-qPCR to detect the expression of 5′UTR, ORF1, and ORF2 of L1, LTR, gag, pol, and env of ERV6 and SINE, respectively. b Sense expression of ORF1 and ORF2, and antisense expression of 5′UTR of L1D in tissues and cells. c Sense and antisense expression of SINEA in tissues and cells. d Sense expression of gag, pol, and env of ERV6, and antisense expression of LTR of ERV6 in tissues and cells