Figure 5.

Mean transit time of plasma through cortical microcirculation. (a) MTT increased post-CA at 30 and 60 min vs. sham (*p < 0.05). Each line represents an individual rat, n = 5/group. (b) The cortical pial vasculature of a representative rat: the yellow square represents a region of interest (ROI) on a pial vein, while the white square represents a ROI on a pial artery. The intensity of the fluorescent tracer was repeatedly measured in the same ROIs at baseline, and at 30 and 60 min post-CA. (c) Intensity-time curves and γ fit curves at baseline, 30, 60 min post-CA (left to right). MTT increased at 30 and 60 min post-CA, indicated by the black arrows. (d) The time-to-peak (TPP) for each pixel on the pia layer was calculated and normalized to mean arterial TPP. Increased MTT was observed at 30, 60 min post-CA in most of the pial veins, indicated by brighter color.