Figure 1.

Raman imaging reveals spatial distribution and relative abundance of major biochemical components throughout single cells. (A) Regression model reference spectra with tentative vibrational assignments as indicated (a-k). (B) Computational algorithm outline for Raman data processing and interpretation methodology; reflected brightfield image of scan position. Scale bar: 5 μm. (C) Pseudo-color ternary scale for visualization of component signals (%) throughout Raman images. (D) Compiled Raman image results for a single cell; spectra were processed and interpreted on a per-pixel basis as described in the methods. Scale bar: 5 μm. (E) 3D surface plot with unexplained signals (%) on the z-axis; calculated R²>0.9 for most pixels of cell image (R² = 0.9 is equivalent to 10% unexplained).