Table 2.

Linearity, inaccuracy, and imprecision characteristics.

				Intra-assay (n = 6)			Inter-assay (n = 18)
Assay range (μg/mL)		Linearity (R2)	QC level	Mean (μg/mL)	Imprecision (% CV)	Inaccuracy (% Dev)	Mean (μg/mL)	Imprecision (% CV)	Inaccuracy (% Dev)
GBP	0.1–100	0.9988	LLOQ	0.110	8.22	14.3	0.120	8.91	17.8
			Low	0.280	7.30	−7.89	0.310	14.9	3.65
			Mid	34.0	2.16	13.2	33.4	3.77	11.4
			High	91.1	5.63	7.20	89.9	4.35	5.72
LTG	0.1–100	0.9998	LLOQ	0.120	7.96	16.0	0.120	7.92	19.7
			Low	0.280	4.54	−8.06	0.310	9.88	2.91
			Mid	33.2	4.71	10.6	32.8	6.17	9.19
			High	97.0	7.40	14.2	94.7	6.01	11.4
LEV	0.1–100	0.9992	LLOQ	0.100	13.2	0.00	0.110	17.3	13.6
			Low	0.330	8.56	9.67	0.340	6.48	15.0
			Mid	31.2	2.18	3.95	31.3	2.02	4.37
			High	85.2	1.14	0.210	83.0	2.49	−2.40
MHD	0.1–100	0.9999	LLOQ	0.120	4.73	15.5	0.120	4.33	16.2
			Low	0.330	3.75	10.2	0.330	2.82	10.7
			Mid	31.2	1.44	4.07	31.2	1.66	3.95
			High	89.2	2.07	4.99	89.0	1.87	4.70
ZNS	0.1–100	0.9998	LLOQ	0.120	7.50	18.3	0.120	11.1	18.4
			Low	0.320	4.17	5.28	0.330	6.83	10.4
			Mid	33.7	4.36	12.5	33.1	4.40	10.5
			High	91.3	2.83	7.46	94.2	3.54	10.8