Figure 7.

Pharmacological inhibition of PI3Kα reduces RNMT dependency. (a,b) T47D and MCF7 cells were incubated with 50 nM GDC-0941 or vehicle control (veh), and transfected with RNMT siRNA1 for 72 h. (c,d) T47D and JIMT cells were incubated with 15 nM BYL719 or vehicle control (veh), and transfected with RNMT siRNA1 for 72 h. (a,c) Cell extracts were analysed by western blot. (b,d) Average cell number and standard deviation for at least three independent experiments are presented. Statistical significance was assessed using ANOVA followed by Dunnett's multiple comparison tests. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001 indicates p-values relative to control indicated.