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. 2019 May 1;11:3779–3790. doi: 10.2147/CMAR.S185438

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© 2019 Yang et al. This work is published and licensed by Dove Medical Press Limited

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Influence of FOXM1 on β-catenin expression in nuclear and cytoplasm.

Notes: DLD-1 and HCT116 cells were transfected with FOXM1 plasmids or siRNAs for 48 hours. The expressions of β-catenin in nuclear and cytoplasm were examined by western blots. (A) Cytoplasmic and nuclear levels of β-catenin were examined in DLD-1 after transfection with FOXM1 plasmids. (B) Cytoplasmic and nuclear levels of β-catenin were examined in HCT116 after transfection with FOXM1 siRNAs. β-actin was used as the loading control for cytoplasmic protein. Lamin B1 was used as the loading control for nuclear proteins. Each experiment was repeated at least three times. **P<0.01.

Abbreviations: si-control, control-siRNA; si-FOXM1, FOXM1 siRNA.