Figure 5.
Anti-H1 antibody profile of mice immunized with, PBS, BLANK-NP, H1 and H1-NP.
Notes: Mice (n=8) were bled on days 14, 28, and 42 postimmunization, and anti-H1 specific total IgG, IgG1, and IgG2a end-point titers were determined by ELISA. (A–C) Anti-H1 IgG end-point titers of each mouse group on days 14, 28, and 42, respectively; (D–F) anti-H1 IgG1 end-point titers of each mouse group on days 14, 28, and 42, respectively; (G–I) anti-H1 IgG2a end-point titers of each mouse group on days 14, 28, and 42, respectively. Each point represents the mean of triplicate values of individual mice, and the bars represent means ± SEM of all mice in a group.
Abbreviations: PLGA, poly(lactic-co-glycolic acid); NPs, nanoparticles; NS, not significant.