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. 2019 May 1;14:3129–3143. doi: 10.2147/IJN.S172391

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© 2019 Malik et al. This work is published and licensed by Dove Medical Press Limited

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Cytokine release in splenocyte-culture supernatant upon in vitro stimulation with H1 antigen.

Notes: Six weeks after immunization, spleens were isolated from mice (n=3) from each group and cultured. IFNγ, TNFα, IL4, and IL10 levels were measured in H1 (10 µg/mL) in vitro–stimulated spleen cell–culture supernatant after 72 hours. Concentrations of (A) IFNγ, (B) TNFα, (C) IL4, and (D) IL10 were examined by ELISA. Concentration values expressed as means ± SE of three mice for each group of mice.

Abbreviation: NPs, nanoparticles.