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. 2019 Apr 29;13:134. doi: 10.3389/fncel.2019.00134

FIGURE 1.

FIGURE 1

Electrophysiological and behavioral responses evoked by coffee furanone for different insect orders. (A) Left: phylogeny of insects for which coffee furanone (CF) elicited antennal activity. Right graphs indicate electroantennogram responses of respective species to 0.5 s odor stimulations of coffee furanone (red bar) at concentration of 10-1 g/mL and 0.5 L/min. a, Lepismachilis γ-signata (image from Missbach et al., 2014), b, silverfish c, grasshopper d, Blattella germanica e, Xylotrechus quadripes f, Apis dorsata g, Helicoverpa armigera h, Drosophila melanogaster. (B) Drosophila melanogaster, CantonS (Cs) strain, Blattella germanica tested at 10-1 g/mL dilution, and Aedes aegypti tested with 100 g/mL of CF in Y-tube olfactometry assays (CF = coffee furanone; ∗∗p value < 0.005, ∗p value < 0.05, Chi-square test). (C) Drosophila melanogaster, CantonS (Cs) strain tested to known attractant 2, 3–butanedione (BTD) at a concentration of 10-2 g/mL; Aedes aegypti and Blattella germanica tested to known repellent citronellal at 10-2 g/mL. (D) Cs and Orco-/- flies (C = control), tested for various concentrations of coffee furanone.