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. 2019 Apr 26;10:820. doi: 10.3389/fmicb.2019.00820

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Copyright © 2019 Masum, Siddiqa, Ali, Zhang, Abdallah, Ibrahim, Qiu, Yan and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Live/dead cell staining analysis of Acidovorax oryzae strain RS-2 cells exposed to 20 μg/ml of silver nanoparticles (AgNPs) for 6 h. Staining were carried out using live/dead BacLight bacterial viability kit (Invitrogen, Carlsbad, CA, United States), and visualized by fluorescence microscopy. Green fluorescence is representative of live bacteria with intact membranes, while red fluorescence is representative of dead bacteria. (A) Live bacteria in negative control (without AgNPs); (B) Dead bacteria in negative control (Heat); (C) Bacteria in AgNPs treatment.