FIGURE 12.

Effect of silver nanoparticles (AgNPs) mediated Phyllanthus emblica fruit extract on the secretion of Hcp protein in Acidovorax oryzae strain RS-2. (A) ELISA of Hcp protein at different concentrations; (B) Standard curve, the negative reaction (P/N value < 1.5); (C) ELISA measurement of Hcp secreted by strain RS-2. The positive reaction (P/N value ≥ 1.5); The purified Hcp-His fusion protein and His protein were used as the positive and the negative control, respectively. The experiment was conducted three times with three replicates.