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. Author manuscript; available in PMC: 2020 May 2.
Published in final edited form as: J Phys Chem B. 2019 Apr 18;123(17):3706–3710. doi: 10.1021/acs.jpcb.9b03132

Figure 2.

Figure 2.

15N relaxation and cross-correlation measurements of for the imidazole Nδ1H groups of His25 (blue) and His53 (red) at the 1H frequency of 600 MHz. (A, B) Data for measurements of the 15N CSA / 15N-1H DD cross-correlation rates ηxy (Panel A) and ηz (Panel B). Solid lines show the best-fit curves obtained with Icross/Iauto = tanh(ητ).15 (C) Data for measurements of the 15N longitudinal relaxation rates R1. (D) F1 slices of the spectra recorded with (black) and without (magenta) 1H saturation in the heteronuclear [1H-]15N NOE experiment.