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. Author manuscript; available in PMC: 2019 May 6.
Published in final edited form as: Science. 2009 Mar 12;324(5927):654–657. doi: 10.1126/science.1170803

Figure 1. Circadian oscillation of NAD+ is controlled by the clock.

Figure 1

(A and B) Cellular NAD+ was extracted from serum-entrained MEFs derived from wild type (wt) (A), clock/clock mutant mice (c/c) and Cry1/Cry2 double KO mice (cry) (B) at indicated time points and analyzed by LC/MSn. Three independent experiments were performed and representative results are shown. All data are means ± SEM of three independent samples. (C) Average NAD+ levels in wt, c/c and cry MEFs. Data are shown as means ± SEM of >50 independent samples. (D) Cellular nicotinamide (NAM) was extracted from serum-entrained MEFs derived from wt, c/c and cry mice at indicated time points and analyzed by LC/MSn. (E) Average NAM levels in wt, c/c and cry MEFs. Data are shown as means ± SEM of >50 independent samples.