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. Author manuscript; available in PMC: 2019 May 6.
Published in final edited form as: Science. 2009 Mar 12;324(5927):654–657. doi: 10.1126/science.1170803

Figure 4. NAMPT modulates the circadian clock.

Figure 4

(A) Per2 and Dbp gene expression levels in serum-entrained MEFs treated with 10 nM FK866 or EtOH as control (solvent: ctrl) were analyzed by q-PCR. Highest value for each gene in EtOH treated MEFs was set to 1. All data are means ± SEM of three independent samples. (B) BMAL1 Lys537 acetylation profile in serum-entrained MEFs either treated with 10 nM FK866 or EtOH. Extracts prepared from indicated time points and BMAL1 acetylation was monitored (7). Input samples were probed with anti-BMAL1 and anti-GAPDH antibodies. (C) Scheme of the transcription-enzymatic interplay by which the circadian machinery governs the intracellular levels of NAD+. The NAD+-dependent deacetylase SIRT1 is thereby controlling the oscillatory synthesis of its own coenzyme.