KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| rabbit anti-UCP1 polyclonal antibody | Chemicon | #3046 |
| Deposited Data | ||
| Mouse brown adipose, high fat diet | http://circadiomics.ics.uci.edu/ | MOUSE BROWN ADIPOSE DYAR 2018 HIGH-FAT |
| Mouse brown adipose, normal chow | http://circadiomics.ics.uci.edu/ | MOUSE BROWN ADIPOSE DYAR 2018 NORMAL-CHOW |
| Mouse liver, high fat diet | http://circadiomics.ics.uci.edu/ | MOUSE LIVER METABOLOME ECKEL-MAHAN 2013 HIGH-FAT |
| Mouse liver, normal chow | http://circadiomics.ics.uci.edu/ | MOUSE LIVER METABOLOME ECKEL-MAHAN 2013 NORMAL-CHOW |
| Mouse muscle, high fat diet | http://circadiomics.ics.uci.edu/ | MOUSE MUSCLE DYAR 2018 HIGH-FAT |
| Mouse muscle, normal chow | http://circadiomics.ics.uci.edu/ | MOUSE MUSCLE DYAR 2018 NORMAL-CHOW |
| Mouse prefrontal cortex, high fat diet | http://circadiomics.ics.uci.edu/ | MOUSE PREFRONTAL CORTEX NA 2018 HIGH-FAT |
| Mouse prefrontal cortex, normal chow | http://circadiomics.ics.uci.edu/ | MOUSE PREFRONTAL CORTEX NA 2018 NORMAL-CHOW |
| Mouse serum, high fat diet | http://circadiomics.ics.uci.edu/ | MOUSE SERUM ABBONDANTE 2016 HIGH-FAT |
| Mouse serum, normal chow | http://circadiomics.ics.uci.edu/ | MOUSE SERUM ABBONDANTE 2016 NORMAL-CHOW |
| Mouse sperm, high fat diet | http://circadiomics.ics.uci.edu/ | MOUSE SPERM DYAR 2018 HIGH-FAT |
| Mouse sperm, normal chow | http://circadiomics.ics.uci.edu/ | MOUSE SPERM DYAR 2018 NORMAL-CHOW |
| Mouse suprachiasmatic nucleus, high fat diet | http://circadiomics.ics.uci.edu/ | MOUSE SUPRACHIASMATIC NUCLEUS NA 2018 HIGH-FAT |
| Mouse suprachiasmatic nucleus, normal chow | http://circadiomics.ics.uci.edu/ | MOUSE SUPRACHIASMATIC NUCLEUS NA 2018 NORMAL-CHOW |
| Mouse white adipose, high fat diet | http://circadiomics.ics.uci.edu/ | MOUSE WHITE ADIPOSE DYAR 2018 HIGH-FAT |
| Mouse white adipose, normal chow | http://circadiomics.ics.uci.edu/ | MOUSE WHITE ADIPOSE DYAR 2018 NORMAL-CHOW |
| Raw “origscale” 24-h metabolomics intensity data from 8 different murine tissues under chow or high fat diet | doi:10.17632/zndtmk8xc6.1 | Table_S4.xlsx |
| Diets | ||
| Vivarium Chow- Prolab RMH 2500 | Lab Supply | 5P14 |
| High Fat Diet | Research Diets | D12492 |
| Mice | ||
| C57BL/6J | The Jackson Laboratory | 000664 |
| Plasmids | ||
| pcDNA3.1 | Invitrogen | V79020 |
| Mouse UCP1 ORF (NM009463) cloned into pcDNA3.1 | Martin Jastroch | n/a |
| Software | ||
| MATLAB and Statistics Toolbox R2016b | Mathworks | https://www.mathworks.com/products/new_products/release2016b.html |
| JTK_CYCLE | Hughes et al., 2010 | https://github.com/mfcovington/jtk-cycle |
| BIO_CYCLE | Agostinelli et al., 2016 | http://circadiomics.igb.uci.edu/static/downloadables/BioCycle.tar.gz |
| Cell lines | ||
| HEK293 | Sigma | 85120602 |
| Chemicals | ||
| TRIzol reagent | Invitrogen | 15596018 |
| SsoAdvanced Universal SYBR Green | Bio-Rad | Cat#4385618 |
| iScript cDNA reverse transcription kit | Bio-Rad | Cat#1708891 |
| DMEM, 4,500 mg/L glucose, + L-glutamine, -pyruvate | Gibco | 11965092 |
| FBS | Biochrom | S0115 |
| Gentamycin | Gibco | 15750060 |
| Amphotericin B | Sigma | A2942 |
| Superfect® Transfection Reagent | Qiagen | 301305 |
| Geneticin | Gibco | 10131035 |
| Fat-free BSA | Sigma | A3803 |
| GDP | Sigma | G7127 |
| GMP | Sigma | G8377 |
| IMP | Sigma | I4375 |
| Oligonucleotides | ||
| See Table S6 for qPCR primers | n/a | n/a |