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. Author manuscript; available in PMC: 2019 May 6.
Published in final edited form as: Nat Struct Mol Biol. 2010 Nov 28;17(12):1414–1421. doi: 10.1038/nsmb.1961

Figure 2. MLL1 governs circadian oscillation of histone modifications and gene expression.

Figure 2

(a) MLL1 deficient MEFs demonstrate impaired CLOCK:BMAL1 mediated transcriptional activities. MLL1 was expressed with or without CLOCK:BMAL1 in MEFs derived from wild type (black) or MLL1-KO (gray) mice. In MLL1-KO MEFs, CLOCK:BMAL1-mediated Dbp and Per2 promoter transactivation was basically abolished, however ectopic expression of MLL1 rescued the expression. (Means ±SEM of seven samples from two independent experiments, *p<0.001). (b) Circadian H3K4me3 is completely impaired in MLL1-KO MEFs. Dexamethasone treated wild type (square) and MLL1-KO (triangle) MEFs were analyzed by ChIP using anti-H3K4me3 (left) or anti-acetylated H3K9/14 antibodies (right). (Means ±SEM of three independent samples, ANOVA p<0.001, and *p<0.05, **p<0.001 at t-test) (c) Circadian rhythmic expression of Dbp and Per2 were dramatically decreased in MLL1-KO MEFs. mRNA was extracted from wild type (black) or MLL1-KO (gray) MEFs after dexamethasone shock and analyzed by quantitative real-time PCR. The amount of highest expression time point was set to 1. (Means ±SEM of three samples, ANOVA p<0.001, and *p<0.05 at t-test)