Table 1.
Primers used with template plasmid pSUB11
| Primer | Sequence, 5′–3′* | Target gene | GenBank |
|---|---|---|---|
| pp190 (fw) | TTTACACTCCTCCTCCTGGAAAGACTGGTGTACCATTTTAGACTACAAAGACCATGACGG | gtgE | AF147699 |
| pp145 (rv) | CGACGGCTTTATTCCCCAGTTTCACCCCATAGCTTCCATATGAATATCCTCCTTAG | ||
| pp180 (fw) | TGGTGGCGGTGCACGTTTTGCCTGTGGTGTCATTGAGAAAGACTACAAAGACCATGACGG | sodCI | AF007380 |
| pp182 (rv) | TTTTCCACTGATGCTGACAGTGACGTCAAAGCTGGAACCTCATATGAATATCCTCCTTAG | ||
| pp193 (fw) | CAATACAGGCATTCTGCAGAGGTTTTCCCGGATGAAGACTACAAAGACCATGACGG | gogC | — |
| pp128 (rv) | TGGTTTTTGTCACGCGTTTTATGGATAGTTTTCTGCCATATGAATATCCTCCTTAG | ||
| pp189 (fw) | GGCGGTGGTGCACGAATCGCCTGTGGCGTTATAAAAGACTACAAAGACCATGACGG | sodCIII | AF254764 |
| pp167 (rv) | GGTAATGCCCTGCCCGTGCTGTACGGCGAAATGCTGCATATGAATATCCTCCTTAG | ||
| pp187 (fw) | AGTTGGTTGGGATAAATATAAGCCTAAAAATAGAAATCGTGACTACAAAGACCATGACGG | gogB | AF254761 |
| pp138 (rv) | TACTAACAACCACAAACCTGAGACCAATTCAGTTGCTTCACATATGAATATCCTCCTTAG | ||
| pp188 (fw) | TACCTTTAAAAAGCAAAAATATTCCTTAATAGGTAAAATGGACTACAAAGACCATGACGG | gtgB | AF254763 |
| pp140 (rv) | AAGAAAACCACCTCACCCTCATAACTCAGTAAGCGTCCCGCATATGAATATCCTCCTTAG | ||
| pp215 (fw) | TGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGGACTACAAAGACCATGACGG | cat | J01841 |
| pp136 (rv) | CCATTCATCCGCTTATTATCACTTATTCAGGCGTAGCACCCATATGAATATCCTCCTTAG | ||
| pp194 (fw) | AGCGTTCCTCGCCATTCTGCATGTCGGTAAAGACAATAAAGACTACAAAGACCATGACGG | lepA | X54933 |
| pp169 (rv) | GCAAACATGTTCGCCATGCCAACTCCTTAGGGATTATTTACATATGAATATCCTCCTTAG | ||
| pp220 (fw) | ACAGGCGTACGCCTGAGTCGTATCGGCGGTATTCACGACTACAAAGACCATGACGG | lepB | X54933 |
| pp126 (rv) | GCCGTGGCGACCAAACGGCGCCAACCTAAAACTTCGCATATGAATATCCTCCTTAG |
*
Primers were designed to anneal to the beginning of the 3xFLAG-coding sequence (fw) and on the opposite side from the KnR cassette (rv) of template plasmid pSUB11 (in italics), and carry extensions homologous to the last 36–40 nts of the target gene coding sequence (fw) and to a region downstream from it (rv). fw, forward; rv, reverse.