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. Author manuscript; available in PMC: 2020 Mar 21.
Published in final edited form as: Cell Chem Biol. 2019 Jan 10;26(3):331–339.e3. doi: 10.1016/j.chembiol.2018.11.011

Figure 1. Development of phenotypic small molecule immunomodulator discovery platform.

Figure 1.

(A) 384-well plate based immune- and cancer-cell co-culture system to model human cancer-immune microenvironment. (B) Image-based readout for monitoring cancer cell proliferation. (C) Biochemical-based readout for measuring cell viability using fluorescence signal of resofurin from viable cells. (D) principle for screening of small molecules with capability to induce immune cell-mediated killing of cancer cell. (E) PBMC-dose dependent killing curves for KRAS WT and G12V isogenic SW48 cell lines. The data are presented as mean ± SEM from four independent experiments. (F) AUC analysis of PBMC-dose dependent killing curves of 3 pairs of KRAS WT and G12V isogenic cell lines. Each dot represents one cell line and the line indicate the mean. **p<0.01, *p<0.05. See also Figure S1 and S2.