Skip to main content
. Author manuscript; available in PMC: 2019 Oct 8.
Published in final edited form as: Nat Cell Biol. 2019 Apr 8;21(5):592–602. doi: 10.1038/s41556-019-0307-4

Table 1.

Molar ratios in the CAP/actin complex.

Mole percentage of indicated protein compared to CAP* CAP1 CAP2 CAP1&2 BIF
Actin Avg 82.5% 88.1% 90.5% 106.8%
STD 9.4% 11.7% 10.7% 4.3%
N 6 24 2 3
Untagged CAP Avg 16.8% 16.3% 16.5% NA
STD 14.3% 12.4% 15.0% NA
N 2 20 3 NA

Calculated from densitometry of Coomassie-stained SDS-PAGE. CAP1, CAP2, and CAP1&2 = purified GFP-fusion proteins from HEK293 cells. BIF = purified brain inhibitory fraction.

*

represents CAP-GFP (for CAP1, CAP2, and CAP1&2) or CAP (BIF) set to 100%.

N = number of independent protein preparations analyzed.