Table 1.
Mole percentage of indicated protein compared to CAP* | CAP1 | CAP2 | CAP1&2 | BIF | |
---|---|---|---|---|---|
Actin | Avg | 82.5% | 88.1% | 90.5% | 106.8% |
STD | 9.4% | 11.7% | 10.7% | 4.3% | |
N | 6 | 24 | 2 | 3 | |
Untagged CAP | Avg | 16.8% | 16.3% | 16.5% | NA |
STD | 14.3% | 12.4% | 15.0% | NA | |
N | 2 | 20 | 3 | NA |
Calculated from densitometry of Coomassie-stained SDS-PAGE. CAP1, CAP2, and CAP1&2 = purified GFP-fusion proteins from HEK293 cells. BIF = purified brain inhibitory fraction.
represents CAP-GFP (for CAP1, CAP2, and CAP1&2) or CAP (BIF) set to 100%.
N = number of independent protein preparations analyzed.