Figure 5. Loss of GALNT3 Results in the Intracellular Retention of E-Cadherin in the Golgi.

(A) Reduced GALNT3 expression in TS^KI4 cells results in the retention of E-cadherin in the Golgi. Confocal images in TS^WT and TS^KI4 cells infected with a control virus, TS^WT cells infected with Galnt3 shRNA (TS^WTGsh) and TS^KI4 cells expressing human GALNT3 (TS^KI4Gab) are shown. 4^′,6-Dia midino-2-phenylindole (DAPI; blue), E-cadherin (green), and Giantin (Golgi) (red). Arrowheads indicate co-localization of E-cadherin and Giantin in the Golgi.

(B) HDAC6 knockdown in TS^KI4 cells results in the partial restoration of E-cadherin to the cell surface. Confocal images in TS^WT cells and TS^KI4 cells expressing control shRNA or TS^KI4 cells expressing Hdac6 shRNA (TS^KI4H6sh) are shown. Arrows show the area of enlarged insets. Arrowheads indicate punctate intracellular E-cadherin localization.

(C) GALNT3 knockdown in HMECs results in the intracellular trapping of E-cadherin. Confocal images for HMECs infected with a control shRNA or two independent Galnt3 shRNAs (HMEC^Gsh1 and HMEC^Gsh2) are shown.

(A–C) Images are representative of three independent experiments. White bar represents 50 μm.