KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Alexa Fluor Phalloidin 594 | Thermo Fisher Scientific | Cat#A12381; RRID: AB_2315633 |
| Alexa Fluor 488-conjugated Streptavidin | Jackson ImmunoResearch Labs | Cat#016-540-084; RRID: AB_2337249 |
| Donkey anti-mouse peroxidase conjugate | Jackson ImmunoResearch Labs | Cat#715-035-151; RRID: AB_2340771 |
| Donkey anti-rabbit peroxidase conjugate | Jackson ImmunoResearch Labs | Cat#711-035-152; RRID: AB_10015282 |
| Goat anti-mouse polyclonal Daylight 488 | Thermo Fisher Scientific | Cat#35503; RRID: AB_1965946 |
| Goat anti-rabbit polyclonal Daylight 488 | Thermo Fisher Scientific | Cat#35553; RRID: AB_1965947 |
| Mouse monoclonal anti-claudin-6 | Santa Cruz Biotechnology | Cat#sc-393671 |
| Mouse monoclonal anti-E-cadherin | BD Biosciences | Cat#610181; RRID: AB_397580 |
| Mouse monoclonal anti-Flag | Thermo Fisher Scientific | Cat#MA1–91878; RRID: AB_1957945 |
| Mouse monoclonal anti-GAPDH | Thermo Fisher Scientific | Cat#AM4300; RRID: AB_2536381 |
| Mouse monoclonal anti-Tubulin | Santa Cruz Biotechnology | Cat#sc-53646; RRID: AB_630403 |
| Mouse monoclonal anti-Tubulin | Sigma-Aldrich | Cat#T6793; RRID: AB_477585 |
| Mouse monoclonal anti-Vinculin | Abcam | Cat#ab18058; RRID: AB_444215 |
| Rabbit polyclonal Alexa 594 | Cell Signaling Technology | Cat#8889; RRID: AB_2716249 |
| Rabbit monoclonal anti-Alpha 1 catenin | Abcam | Cat#ab51032; RRID: AB_868700 |
| Rabbit monoclonal anti-Delta 1 catenin | Abcam | Cat#ab92514; RRID: AB_10565040 |
| Rabbit monoclonal anti-E-cadherin | Abcam | Cat#ab212059 |
| Rabbit monoclonal anti-H2BK5Ac | Active Motif | Cat#39123; RRID: AB_2615079 |
| Rabbit monoclonal anti-HDAC6 | Cell Signaling Technology | Cat#7612; RRID: AB_10889735 |
| Rabbit monoclonal anti-IgG | Abcam | Cat#ab172730; RRID: AB_2687931 |
| Rabbit monoclonal anti-Lamin B1 | Abcam | Cat#ab133741; RRID: AB_2616597 |
| Rabbit polyclonal anti-ERK2 | Santa Cruz Biotechnology | Cat#sc-154; RRID: AB_2141292 |
| Rabbit polyclonal anti-GALNT3 | Abgent | Cat#AP9208C; RRID: AB_10612485 |
| Rabbit polyclonal anti-Giantin | Abcam | Cat#ab24586; RRID: AB_448163 |
| Rabbit polyclonal anti-HDAC6 | Millipore Sigma | Cat#07–732; RRID: AB_441966 |
| Rabbit polyclonal anti-IgG | Abcam | Cat#ab171870; RRID: AB_2687657 |
| Rabbit polyclonal anti-ZO-1 | Thermo Fisher Scientific | Cat#40–2200; RRID: AB_2533456 |
| Biotinylated Vicia Villosa Lectin | Vector Laboratories | Cat#B-1235; RRID: AB_2336855 |
| Peroxidase-streptavidin | Jackson ImmunoResearch Labs | Cat#016-030-084; RRID: AB_2337238 |
| Bacterial and Virus Strains | ||
| Mouse Galnt3 shRNA (Gsh1) | Open Biosystems | TRCN0000055098 |
| Mouse Galnt3 shRNA (Gsh2) | Open Biosystems | TRCN0000055099 |
| Human Galnt3 shRNA (Gsh1) | Dharmacon | TRCN0000035456 |
| Human Galnt3 shRNA (Gsh2) | Dharmacon | TRCN0000035458 |
| Entry Vector Human Galnt3 | Vidal Human ORFeome (Version 5.1) | Clone# 55179 BCII3565 in pDONR223 |
| pENTR-Cdh1 | Cachat et al., 2014 | Addgene Plasmid#49776; RRID: Addgene_49776 |
| Lentiviral FLAG tagged destination vector | Kind Gift from Dr. Gary Johnson (Jordan et al., 2013) | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| 2-mercaptoethanol (BME) | Thermo Fisher Scientific | Cat#31350010 |
| Agarose bound Vicia Villosa Lectin beads | Vector Laboratories | Cat#AL-1233; RRID: AB_2336854 |
| Enzyme Free Cell Dissociation Solution PBS Based (1X) | Millipore Sigma | Cat#S-014-C |
| Fetal Bovine Serum-GIBCO | Thermo Fisher Scientific | Cat#10437–028 |
| Hyclone Glutamine | Thermo Fisher Scientific | Cat#SH3003401 |
| Heparin | Sigma-Aldrich | Cat#H3149–10KU; CAS: 9041-08-1 |
| Hyclone Penicillin-Streptomycin | Thermo Fisher Scientific | Cat#SV30010 |
| Recombinant Human FGF4 | Peprotech | Cat#100–31 |
| Recombinant Protein A Sepharose 4B | Thermo Fisher Scientific | Cat#101141 |
| Sodium pyruvate | Thermo Fisher Scientific | Cat#SH30239.01 |
| Trypsin (0.05%) | Thermo Fisher Scientific | Cat#SH30236.01 |
| Insulin, Human Recombinant Zinc Solution | Thermo Fisher Scientific | Cat#12585014 |
| Critical Commercial Assays | ||
| iTaq Universal SYBR Green Supermix | Bio-Rad | Cat#1725125 |
| Rneasy Plus Mini Kit | QIAGEN | Cat#74134 |
| Rneasy Micro Kit | QIAGEN | Cat#74004 |
| High capacity cDNA Reverse Transcription Kit | Thermo Fisher Scientific | Cat#4368813 |
| Gateway LR Clonase II Enzyme mix | Invitrogen | Cat#11791 −020 |
| Corning BioCoat Matrigel Invasion Chamber: With GFR Matrigel Matrix | Thermo Fisher Scientific | Cat#08-774-193 |
| SsoAdvanced Universal SYBR Green Supermix | Bio-Rad | Cat#1725274 |
| GeneJet Plasmid Miniprep Kit | Thermo Fisher Scientific | Cat#K0502 |
| Transwells 6.4mm with 0.4 mm pore polycarbonate | Corning | Cat#3413 |
| Transwells 6.4mm with 0.4 mm pore polyethylene terephthalate | Corning | Cat#353095 |
| Q5 Site-Directed Mutagenesis Kit | New England BioLabs | Cat#E0554S |
| Experimental Models: Cell Lines | ||
| Human: HEK293T | Kind Gift from Dr. Gary Johnson | N/A |
| Human: Human Mammary Epithelial cells | Kind Gift from Dr. Gary Johnson | N/A |
| Human: SUM159 Claudin-low breast cancer cells | Kind Gift from Dr. Gary Johnson | N/A |
| Mouse: MAP3K4 KI4 Trophoblast stem cells | Abell et al., 2009 | N/A |
| Mouse: Wild-type Trophoblast stem cells | Abell et al., 2009 | N/A |
| Experimental Models: Organisms/Strains | ||
| 129/SvEv strain Wild-type Females | Abell et al., 2005, 2009 | N/A |
| Oligonucleotides | ||
| See Table S1 for primer sequences | This paper | Table S1 |
| Software and Algorithms | ||
| Bio-Rad CFX Maestro | CFX Manager (Version 3) | http://www.bio-rad.com/en-us/product/cfx-maestro-software-for-cfx-real-time-pcr-instruments?ID=OKZP7E15 |
| BioTek Lionheart FX (Live cell and VVL quantitation) | BioTek Gen5 (Version 3.2) | https://www.biotek.com/products/software-robotics/ |
| Easeq | Lerdrup et al., 2016 | https://easeq.net/ |
| GraphPad Prism (Plot Graphs and Statistical analysis) | Graph Pad Prism Software (Version 7) | https://www.graphpad.com/scientific-software/prism/; RRID: SCR_002798 |
| NIS-Elements Advanced Research | NIS-Elements AR software (Version 4.51.00) | https://www.microscope.healthcare.nikon.com/products/software; RRID: SCR_014329 |
| Stats package in R | R Development Core Team (2013) | http://www.R-project.org/; RRID: SCR_001905 |
| Other | ||
| EmbryoMax Acidic Tyrode’s Solution | Millipore Sigma | Cat#MR-004-D |
| Hyclone Dulbecco’s Modified Eagles Medium | Thermo Fisher Scientific | Cat#SH3024301 |
| Ham’s F12 Nutrient Mix | Thermo Fisher Scientific | Cat#11765047 |
| HuMEC Basal Serum-Free Medium (1X) | Thermo Fisher Scientific | Cat#12753018 |
| HuMEC Supplement Kit | Thermo Fisher Scientific | Cat#12755013 |
| KSOM Powdered Embryo Culture Medium | Millipore Sigma | Cat#MR-020P-5D |
| EmbryoMax KSOM+AA with D-Glucose and Phenol Red | Millipore Sigma | Cat#M-121-D |
| EmbryoMax M2 Medium (1X), liquid, with Phenol Red | Millipore Sigma | Cat#MR-015-D |
| Hyclone RPMI-1640 medium (1X) | Thermo Fisher Scientific | Cat#SH3002701 |