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. 2019 Feb 15;36(5):919–929. doi: 10.1093/molbev/msz018

Fig. 2.

Fig. 2.

Natural variation at the Or22 locus determines the ab3A neuronal response phenotype. (a–d) Response profiles for flies expressing UAS-Or22 transgenes in ab3A neurons lacking their endogenous receptor (Dobritsa et al. 2003). (a) Expression of Or22a1 results in an ab3A-1 phenotype (comparing to fig. 1a, Spearman rank correlation, P < 0.001). (b) Or22ab results in an ab3A-2 phenotype (comparing to fig. 1d, P < 0.001). (c) Or22a3 results in an ab3A-1 phenotype (comparing to fig. 1a, P < 0.001). (d) Or22b3 results in an ab3A-3 phenotype (comparing to fig. 1f, P < 0.001). Data are represented as the mean ± SEM, n = 6 for all recordings. Paraffin oil is the solvent control. (e) Or22a mRNA transcripts are not detected in Innisfail 2 antennal cDNA preparations. Plasmid DNA template control showing that Or22a primers do not amplify Or22b. NTC, no template control for Or22a primers. Or22 primers (not specific to Or22a or Or22b) detect Or22 expression in the same cDNA prep as lane 1.