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Measurements of free [Ca2+] in the synaptic terminal. (A) Responses to depolarizations lasting 20 ms (bold black traces) and 500 ms (thin red traces) in a terminal dialyzed with 0.4 mM EGTA and 0.1 mM fura-2. (B) Responses from a terminal dialyzed with 2 mM EGTA and 0.1 mM fura-2. The corresponding Ca2+ currents are shown below. (C) Responses to 20-ms stimuli from A and B on expanded scales.
