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. 2001 Dec 11;98(26):15294–15299. doi: 10.1073/pnas.261560998

Figure 2.

Figure 2

NO hyperpolarizes astrocytic mitochondria, but depolarizes neuronal mitochondria. Cell suspensions (2 × 106 cells per ml) were incubated at 37°C in buffered Hanks' solution either in the absence (control) or presence of DETA-NO (0.5 mM) for the indicated times. In this buffer, 0.5 mM DETA-NO was found to generate a constant NO concentration of 1.4 μM. For Δψm measurements, cell suspensions were centrifuged, and the pellet was resuspended in buffered Hanks' solution containing JC-1 (3 μM) and incubated for a further 10-min at 37°C. Data acquisition was performed in a FACScalibur flow cytometer as detailed in Materials and Methods. Data are expressed as a percentage, with the red/green fluorescence ratio values of untreated cells (2.98 ± 0.10 for neurons, 3.11 ± 0.20 for astrocytes) considered as 100% and the red/green fluorescence ratio values of FCCP (5 μM)-treated cells (0.68 ± 0.04 for neurons, 0.84 ± 0.05 for astrocytes) considered as 0%. *, P < 0.05 versus appropriate control values.