Figure 3.
NO-dependent glycolytic activation determines mitochondrial membrane potential. Cell suspensions (2 × 106 cells per ml) were incubated at 37°C in buffered Hanks' solution either in the absence (control) or presence of DETA-NO (0.5 mM) for 60 min. For glucose-deprivation experiments (Glc depr), both the preincubation (45 min) and incubation (60 min) periods were carried out in glucose-free Hanks' solution. Under these conditions, the astrocytic content of glycogen was shown to be depleted. Where indicated, cells were incubated in the presence of 20 mM fructose throughout. ATP and lactate concentrations, as well as Δψm, were determined as described in the legends to Figs. 1 and 2. *, P < 0.05 versus appropriate control values.