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. 2019 May 6;15(5):e1008084. doi: 10.1371/journal.pgen.1008084

Fig 8. NR1D1 is required for the upregulated Ulk1 expression in Stra8-deficient testes.

Fig 8

(A) In situ hybridization of Ulk1 in testicular cross sections from age-matched wild-type and Stra8-deficient testes at 10 d.p.p.. (B) qRT-PCR analysis of Ulk1 expression relative to β-actin level in undifferentiated (c-Kit-negative integrin α6-high) and differentiating (c-Kit-positive integrin α6-low) spermatogonia isolated from juvenile wild-type and Stra8-deficient testes by FACS. Data represent mean ± SD; n = 3 mice per group; *P < 0.05 (Student’s t test). (C) Left panel, schematic of the primer sets targeting RORE sites in the mouse Ulk1 promoter for ChIP analysis. Lower, ChIP analysis of NR1D1 binding to the Ulk1 promoter at the RORE sites in mouse testicular lysates at 21 d.p.p.. Graph represent mean ± SD from triplicate PCR reactions. (D) qRT-PCR analysis of testicular Ulk1 expression relative to β-actin levels in testes with indicated genotypes. Data represent mean ± SD; n = 3 mice per group; *P < 0.05 (Student’s t test).