Table 2. All Samples QC Results (sorted by specimen storage time).
Sample Name | Input for lib prep (ng) | RIN | DV200 | DV100 | DV50 | Lib size (bp) | Lib yield (ng) | Lib Molarity (nM) | Specimen storage time (Years) | *Resequenced? | % contamination | Gene Expression Cluster (Fig 8) | Gene Count |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
(%) | (%) | (%) | |||||||||||
SEER_020 | 672.3 | 2.3 | 30 | 75 | 98 | 413 | 34.8 | 10.3 | 7 | N | 22.61 | B | 14216 |
SEER_064 | 1000 | 2.3 | 42 | 73 | 90 | 478 | 78 | 18.6 | 7 | Y | 55.47 | C | 13844 |
SEER_041 | 1000 | 2.5 | 11 | 41 | 98 | 428 | 8.28 | 2.4 | 9 | N | 4.69 | D | 4541 |
SEER_042 | 1000 | 2.2 | 39 | 81 | 99 | 334 | 5467.95 | 190 | 9 | Y | 0.38 | B | 4850 |
SEER_043 | 1000 | 2.4 | 14 | 50 | 97 | 421 | 19.8 | 5.9 | 9 | N | 13.31 | D | 6429 |
SEER_044 | 232.4 | 2.7 | NA | NA | NA | 393 | 7.26 | 2.5 | 9 | N | 4.25 | D | 16093 |
SEER_045 | 1000 | 2.4 | 16 | 55 | 98 | 368 | 12.5 | 4.6 | 9 | Y | 9.08 | D | 14766 |
SEER_065 | 1000 | 2.3 | 21 | 64 | 98 | 456 | 3.14 | 0.84 | 9 | Y | 23.83 | D | 60 |
SEER_060 | 747 | 2.6 | 6 | 30 | 94 | 445 | 4.55 | 1.2 | 10 | N | 24.75 | B | 27053 |
SEER_061 | 1000 | 2.2 | 50 | 86 | 100 | 331 | 246 | 91 | 10 | Y | 0.48 | B | 5169 |
SEER_016 | 597.8 | 2.5 | 17 | 60 | 98 | 375 | 985.95 | 363.2 | 12 | N | 39.06 | 15313 | |
SEER_017 | 1000 | 1 | NA | NA | NA | 365 | 3.69 | 1.15 | 12 | N | 2.04 | D | 24435 |
SEER_019 | 1000 | 2.4 | 21 | 64 | 98 | 393 | 207.9 | 66 | 12 | N | 12.3 | B | 22417 |
SEER_021 | 1000 | 2.4 | 18 | 67 | 99 | 337 | 417.45 | 154.9 | 12 | N | 1.22 | B | 9993 |
SEER_052 | 391.05 | 2.4 | 11 | 43 | 96 | 405 | 8.55 | 2.6 | 12 | N | 3.95 | D | 15398 |
SEER_013 | 1000 | 2.1 | 35 | 81 | 99 | 312 | 286.8 | 105 | 13 | Y | 0.51 | B | 17729 |
SEER_014 | 568 | 2.5 | 17 | 66 | 99 | 355 | 10.47 | 3.33 | 13 | N | 3.16 | B | 21893 |
SEER_015 | 1024 | 2.4 | 13 | 56 | 98 | 364 | 456.75 | 159.4 | 13 | N | 31.93 | 5688 | |
SEER_018 | 1000 | 2.1 | 36 | 82 | 100 | 290 | 216.9 | 83 | 13 | Y | 0.08 | C | 21580 |
SEER_022 | 1000 | 2.6 | 4 | 42 | 98 | 372 | 197.55 | 43.4 | 13 | N | 2.06 | C | 20620 |
SEER_023 | 1000 | 2.4 | 5 | 34 | 97 | 371 | 389.1 | 145.1 | 13 | N | 33.26 | 22385 | |
SEER_051 | 1000 | 2.4 | 15 | 56 | 98 | 405 | 9.77 | 3 | 13 | N | 8.39 | D | 9062 |
SEER_012 | 907.5 | NA | 10 | 65 | 99 | 329 | 28.13 | 9.46 | 14 | Y | 1.22 | B | 18627 |
SEER_050 | 1000 | 2.4 | 13 | 59 | 98 | 376 | 21 | 6.6 | 14 | Y | 7.22 | D | 16584 |
SEER_066 | 1000 | 2.4 | 12 | 56 | 99 | 426 | 86.1 | 26 | 14 | N | 21.89 | D | 17 |
SEER_029 | 237.6 | 2.5 | 12 | 35 | 93 | 426 | 24.45 | 7 | 15 | Y | 27.38 | D | 31105 |
SEER_049 | 2000 | 1.7 | NA | NA | NA | 446 | 6.56 | 1.5 | 15 | N | 7.09 | D | 3380 |
SEER_010 | 1000 | 1.1 | NA | NA | NA | 315 | 44.21 | 15.23 | 16 | Y | 0.58 | B | 17485 |
SEER_011 | 682.5 | 2.2 | NA | NA | NA | 411 | 1.11 | 0.3 | 16 | N | 3.76 | 22 | |
SEER_027 | 830.7 | 2.5 | 9 | 51 | 98 | 385 | 84.75 | 27.7 | 16 | N | 9.36 | B | 9056 |
SEER_009 | 369.75 | NA | NA | NA | NA | 353 | 8.58 | 2.65 | 17 | Y | 5.11 | B | 17832 |
SEER_048 | 1000 | 2.4 | 15 | 55 | 97 | 399 | 13.62 | 4.2 | 17 | N | 10.27 | C | 5981 |
SEER_007 | 1000 | 1 | NA | NA | NA | 408 | 5.04 | 1.43 | 18 | Y | 1.71 | C | 42 |
SEER_008 | 4731 | 1.1 | NA | NA | NA | 307 | 211.35 | 82.7 | 18 | Y | 0.08 | D | 8177 |
SEER_025 | 576.2 | 2.5 | 11 | 50 | 97 | 408 | 50.25 | 14.7 | 18 | N | 24.04 | B | 20527 |
SEER_026 | 1000 | 2.5 | 8 | 48 | 97 | 409 | 59.7 | 17.6 | 18 | N | 22.36 | B | 8392 |
SEER_046 | 1000 | 2.4 | 12 | 48 | 98 | 404 | 17.4 | 5.4 | 18 | Y | 9.89 | D | 4703 |
SEER_047 | 1000 | 2.3 | 22 | 67 | 99 | 387 | 23.25 | 7.3 | 18 | N | 10.9 | D | 5145 |
SEER_063 | 1000 | 2.3 | 27 | 77 | 99 | 356 | 124.2 | 41 | 18 | N | 0.49 | B | 10920 |
SEER_006 | 392.2 | NA | NA | NA | NA | 450 | 2.49 | 0.622 | 19 | Y | 12.02 | B | 12652 |
SEER_024 | 926.55 | 2.5 | 12 | 57 | 99 | 360 | 207.75 | 67.9 | 20 | N | 1.04 | C | 22926 |
SEER_028 | 697.2 | 2.5 | 9 | 46 | 96 | 433 | 38.4 | 10.9 | 20 | N | 23.14 | B | 10202 |
SEER_036 | 336.7 | 2.8 | 18 | 59 | 98 | 410 | 8.79 | 2.7 | 21 | N | 9.18 | D | 4115 |
SEER_057 | 1000 | 2.2 | 26 | 68 | 98 | 571 | 5.81 | 1.4 | 21 | N | 24.29 | D | 14391 |
SEER_058 | 1000 | 2.4 | 12 | 51 | 97 | 479 | 4.89 | 1.3 | 21 | Y | 29.53 | D | 27840 |
SEER_059 | 1000 | 2.4 | 12 | 53 | 97 | 520 | 8.3 | 2 | 21 | N | 18.6 | C | 23689 |
SEER_067 | 1000 | 2.3 | 24 | 71 | 99 | 462 | 84.9 | 24 | 21 | N | 40.39 | C | 24 |
SEER_034 | 1000 | 2.2 | 47 | 85 | 98 | 352 | 49.05 | 16 | 22 | N | 5 | C | 9 |
SEER_035 | 716.8 | 2.3 | 33 | 79 | 89 | 294 | 218.25 | 76.6 | 22 | N | 1.99 | B | 4000 |
SEER_056 | 21.6 | NA | 4 | 24 | 92 | 444 | 28.2 | 7.3 | 22 | N | 35.6 | B | 8966 |
SEER_037 | 356.25 | 2.6 | 10 | 47 | 95 | 406 | 6.63 | 2.04 | 23 | N | 4.38 | D | 8645 |
SEER_038 | 1000 | 2.5 | 13 | 55 | 97 | 448 | 6.68 | 1.9 | 23 | N | 8.5 | D | 12 |
SEER_039 | 1000 | 2.5 | 14 | 57 | 98 | 408 | 11.75 | 3.6 | 23 | N | 5.36 | D | 16370 |
SEER_040 | 93.6 | 1.5 | 7 | 38 | 76 | 479 | 15.9 | 4 | 23 | Y | 24.54 | D | 9782 |
SEER_055 | 94.6 | 2.3 | 1 | 11 | 85 | 450 | 30 | 7.6 | 23 | N | 35.71 | B | 2448 |
SEER_005 | 1000 | 1.1 | NA | NA | NA | 382 | 5.07 | 1.53 | 24 | Y | 2.69 | C | 8963 |
SEER_053 | 315.25 | 2.6 | 9 | 36 | 89 | 547 | 11.3 | 2.5 | 24 | N | 18.53 | C | 14759 |
SEER_054 | 1000 | 2.5 | 7 | 46 | 93 | 548 | 8.33 | 1.8 | 24 | Y | 41.29 | B | 4020 |
SEER_062 | 1000 | 2.3 | 22 | 74 | 99 | 344 | 366 | 126 | 24 | N | 0.41 | B | 4152 |
SEER_004 | 1000 | 2.6 | NA | NA | NA | 427 | 4.22 | 1.13 | 25 | N | 8.43 | D | 25 |
SEER_030 | 413.4 | 2.5 | 16 | 54 | 97 | 433 | 27.3 | 7.7 | 25 | Y | 30.66 | D | 10431 |
SEER_031 | 537.95 | 2.5 | 11 | 51 | 97 | 428 | 36.45 | 10.3 | 25 | N | 44.1 | C | 13883 |
SEER_032 | 256.5 | 2.5 | 13 | 49 | 96 | 377 | 84.3 | 26.9 | 25 | N | 6.66 | C | 28255 |
SEER_003 | 1000 | 1.1 | NA | NA | NA | 381 | 4.77 | 1.44 | 27 | N | 1.95 | D | 24 |
SEER_002 | 799.5 | 2.1 | NA | NA | NA | 402 | 3.2 | 0.882 | 29 | N | 6.47 | D | 17 |
SEER_001 | 696.8 | NA | NA | NA | NA | 424 | 3.8 | 0.983 | 31 | Y | 5.31 | B | 60 |
SEER_033 | 440.7 | NA | 21 | 63 | 98 | 417 | 67.35 | 19.6 | no info | Y | 39.79 | D | 3210 |
*Resequenced = one of the 25 samples that were resequenced due to low sequencing yields. NA = Values were not able to be obtained and were not included in statistical analyses.