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. 2019 May 6;14(5):e0216050. doi: 10.1371/journal.pone.0216050

Table 2. All Samples QC Results (sorted by specimen storage time).

Sample Name Input for lib prep (ng) RIN DV200 DV100 DV50 Lib size (bp) Lib yield (ng) Lib Molarity (nM) Specimen storage time (Years) *Resequenced? % contamination Gene Expression Cluster (Fig 8) Gene Count
(%) (%) (%)
SEER_020 672.3 2.3 30 75 98 413 34.8 10.3 7 N 22.61 B 14216
SEER_064 1000 2.3 42 73 90 478 78 18.6 7 Y 55.47 C 13844
SEER_041 1000 2.5 11 41 98 428 8.28 2.4 9 N 4.69 D 4541
SEER_042 1000 2.2 39 81 99 334 5467.95 190 9 Y 0.38 B 4850
SEER_043 1000 2.4 14 50 97 421 19.8 5.9 9 N 13.31 D 6429
SEER_044 232.4 2.7 NA NA NA 393 7.26 2.5 9 N 4.25 D 16093
SEER_045 1000 2.4 16 55 98 368 12.5 4.6 9 Y 9.08 D 14766
SEER_065 1000 2.3 21 64 98 456 3.14 0.84 9 Y 23.83 D 60
SEER_060 747 2.6 6 30 94 445 4.55 1.2 10 N 24.75 B 27053
SEER_061 1000 2.2 50 86 100 331 246 91 10 Y 0.48 B 5169
SEER_016 597.8 2.5 17 60 98 375 985.95 363.2 12 N 39.06   15313
SEER_017 1000 1 NA NA NA 365 3.69 1.15 12 N 2.04 D 24435
SEER_019 1000 2.4 21 64 98 393 207.9 66 12 N 12.3 B 22417
SEER_021 1000 2.4 18 67 99 337 417.45 154.9 12 N 1.22 B 9993
SEER_052 391.05 2.4 11 43 96 405 8.55 2.6 12 N 3.95 D 15398
SEER_013 1000 2.1 35 81 99 312 286.8 105 13 Y 0.51 B 17729
SEER_014 568 2.5 17 66 99 355 10.47 3.33 13 N 3.16 B 21893
SEER_015 1024 2.4 13 56 98 364 456.75 159.4 13 N 31.93   5688
SEER_018 1000 2.1 36 82 100 290 216.9 83 13 Y 0.08 C 21580
SEER_022 1000 2.6 4 42 98 372 197.55 43.4 13 N 2.06 C 20620
SEER_023 1000 2.4 5 34 97 371 389.1 145.1 13 N 33.26   22385
SEER_051 1000 2.4 15 56 98 405 9.77 3 13 N 8.39 D 9062
SEER_012 907.5 NA 10 65 99 329 28.13 9.46 14 Y 1.22 B 18627
SEER_050 1000 2.4 13 59 98 376 21 6.6 14 Y 7.22 D 16584
SEER_066 1000 2.4 12 56 99 426 86.1 26 14 N 21.89 D 17
SEER_029 237.6 2.5 12 35 93 426 24.45 7 15 Y 27.38 D 31105
SEER_049 2000 1.7 NA NA NA 446 6.56 1.5 15 N 7.09 D 3380
SEER_010 1000 1.1 NA NA NA 315 44.21 15.23 16 Y 0.58 B 17485
SEER_011 682.5 2.2 NA NA NA 411 1.11 0.3 16 N 3.76   22
SEER_027 830.7 2.5 9 51 98 385 84.75 27.7 16 N 9.36 B 9056
SEER_009 369.75 NA NA NA NA 353 8.58 2.65 17 Y 5.11 B 17832
SEER_048 1000 2.4 15 55 97 399 13.62 4.2 17 N 10.27 C 5981
SEER_007 1000 1 NA NA NA 408 5.04 1.43 18 Y 1.71 C 42
SEER_008 4731 1.1 NA NA NA 307 211.35 82.7 18 Y 0.08 D 8177
SEER_025 576.2 2.5 11 50 97 408 50.25 14.7 18 N 24.04 B 20527
SEER_026 1000 2.5 8 48 97 409 59.7 17.6 18 N 22.36 B 8392
SEER_046 1000 2.4 12 48 98 404 17.4 5.4 18 Y 9.89 D 4703
SEER_047 1000 2.3 22 67 99 387 23.25 7.3 18 N 10.9 D 5145
SEER_063 1000 2.3 27 77 99 356 124.2 41 18 N 0.49 B 10920
SEER_006 392.2 NA NA NA NA 450 2.49 0.622 19 Y 12.02 B 12652
SEER_024 926.55 2.5 12 57 99 360 207.75 67.9 20 N 1.04 C 22926
SEER_028 697.2 2.5 9 46 96 433 38.4 10.9 20 N 23.14 B 10202
SEER_036 336.7 2.8 18 59 98 410 8.79 2.7 21 N 9.18 D 4115
SEER_057 1000 2.2 26 68 98 571 5.81 1.4 21 N 24.29 D 14391
SEER_058 1000 2.4 12 51 97 479 4.89 1.3 21 Y 29.53 D 27840
SEER_059 1000 2.4 12 53 97 520 8.3 2 21 N 18.6 C 23689
SEER_067 1000 2.3 24 71 99 462 84.9 24 21 N 40.39 C 24
SEER_034 1000 2.2 47 85 98 352 49.05 16 22 N 5 C 9
SEER_035 716.8 2.3 33 79 89 294 218.25 76.6 22 N 1.99 B 4000
SEER_056 21.6 NA 4 24 92 444 28.2 7.3 22 N 35.6 B 8966
SEER_037 356.25 2.6 10 47 95 406 6.63 2.04 23 N 4.38 D 8645
SEER_038 1000 2.5 13 55 97 448 6.68 1.9 23 N 8.5 D 12
SEER_039 1000 2.5 14 57 98 408 11.75 3.6 23 N 5.36 D 16370
SEER_040 93.6 1.5 7 38 76 479 15.9 4 23 Y 24.54 D 9782
SEER_055 94.6 2.3 1 11 85 450 30 7.6 23 N 35.71 B 2448
SEER_005 1000 1.1 NA NA NA 382 5.07 1.53 24 Y 2.69 C 8963
SEER_053 315.25 2.6 9 36 89 547 11.3 2.5 24 N 18.53 C 14759
SEER_054 1000 2.5 7 46 93 548 8.33 1.8 24 Y 41.29 B 4020
SEER_062 1000 2.3 22 74 99 344 366 126 24 N 0.41 B 4152
SEER_004 1000 2.6 NA NA NA 427 4.22 1.13 25 N 8.43 D 25
SEER_030 413.4 2.5 16 54 97 433 27.3 7.7 25 Y 30.66 D 10431
SEER_031 537.95 2.5 11 51 97 428 36.45 10.3 25 N 44.1 C 13883
SEER_032 256.5 2.5 13 49 96 377 84.3 26.9 25 N 6.66 C 28255
SEER_003 1000 1.1 NA NA NA 381 4.77 1.44 27 N 1.95 D 24
SEER_002 799.5 2.1 NA NA NA 402 3.2 0.882 29 N 6.47 D 17
SEER_001 696.8 NA NA NA NA 424 3.8 0.983 31 Y 5.31 B 60
SEER_033 440.7 NA 21 63 98 417 67.35 19.6 no info Y 39.79 D 3210

*Resequenced = one of the 25 samples that were resequenced due to low sequencing yields. NA = Values were not able to be obtained and were not included in statistical analyses.