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. 2001 Dec 18;98(26):15300–15305. doi: 10.1073/pnas.261358698

Figure 5.

Figure 5

Catalytically inactive PLD1 inhibits neurotransmission by decreasing the number of functional presynaptic release sites. (A) Variance to mean amplitude of IPSC ratio (V/Iav) determined 120 min after PLD1(K898R) injection and normalized to value before injection. (B) Using a binomial model for release, consequences on V = f(Iav) were simulated when IPSC amplitude was decreased after p, n, or q reduction. Note the typical graphical signature for each quantal parameter change. (C1) Representative experiment in which IPSC amplitude was reduced by decreasing the external [Ca2+/Mg2+] from 2.1 to low 0.14 (opens symbols). Then, after returning to high [Ca2+/Mg2+], PLD1(K898R) was injected (closed symbols). The solid lines corresponds to the ensemble Iav of the data. (C2) IPSC fluctuations (ΔI) around their mean are calculated by subtracting Iav to each IPSC amplitude value. (D) The corresponding V = f(Iav) plots. (E) Normalized cumulative plots of five similar experiments. Note that V data were illustrated by stepping five data.