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. 2019 Apr 24;15(4):e1008116. doi: 10.1371/journal.pgen.1008116

Fig 6. Diversification of P. guiyangense CRN proteins and their potential contributions to virulence.

Fig 6

(A) Homology network of oomycete CRN proteins. Each protein is represented by a node. An edge joining two nodes represents sequence similarity shared by the two proteins. The red circled clusters (cluster I-VI) represent P. guiyangense specifically expanded CRN proteins. The number on the left of the red node represents P. guiyangense CRN protein ID. For example, 31 represents CRN31. (B) Evaluation of CRN protein toxicity to Spodoptera frugiperda cells (Sf9). The Sf9 cells were transfected with DNA encoding the CRN proteins. The protein toxicity was measured as the remaining number of viable cells compared to the empty vector (EV) negative control. Protein expression was assessed by western blots (S7A Fig). (C-D) Assay of CRN protein toxicity to insect cells (C, mosquito C6/36 cells; D, Sf9 cells) using CRN proteins produced in E. coli cell extracts. CRN protein production was assessed by western blots (S7C Fig). Differences were assessed for significance using Student’s t-test: *, P<0.05 and **, P<0.01.).