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. Author manuscript; available in PMC: 2019 May 6.
Published in final edited form as: Bioconjug Chem. 2017 May 5;28(5):1481–1490. doi: 10.1021/acs.bioconjchem.7b00153

Figure 4.

Figure 4.

A: Binding and intracellular trafficking of targeted (top) and non-targeted (bottom) liposomes in MB49 murine bladder tumor cells. MB49 cells were treated with liposome formulations in serum-free medium for 2 h at 37 °C prior to imaging by confocal microscopy. Targeted liposome composition: 62.5:35:2:0.5 DPPC:Chol:RWFV-PEG2000-DSPE:Cy5.5-DSPE. Non-targeted liposome composition: 62.5:35:2:0.5 DPPC:Chol:mPEG2000-DSPE:Cy5.5-DSPE. B: MB49 cells were treated with targeted liposomes (2 mol% RWFV-PEG2000-DSPE) for 2 h, followed by incubation for additional 0 h (left) or 10 h (right). Acidic lysosomes were stained with LysoTracker® Blue DND-22 and plasma membranes were stained with AlexaFluor® 680 WGA. Arrows indicate regions of coincident LysoTracker and FITC fluorescence, indicating the co-localization of FITC-labeled liposomes within acidic late endosome/lysosome compartments. Liposome diameters: 58 nm. Liposome composition: 62.5:35:2:0.5 DPPC:Chol:RWFV-PEG2000-DSPE:Cy5.5-DSPE.