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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Toxicol Appl Pharmacol. 2018 Sep 22;362:9–19. doi: 10.1016/j.taap.2018.09.026

FIGURE 5. Effects of AEEA and TGF-β1 on extractability, electrophoretic behaviors of type I collagen, and collagen deposition.

FIGURE 5.

A. Western blot analysis was performed on extracts under denaturing and native conditions to determine whether the extractability and the 3D structure of type I collagen may be altered by AEEA and TGF-β1. HTS fibroblasts were treated with AEEA (0 or 50 μM). 4 days after AEEA treatment, both AEEA and TGF-β1 (2.5 ng/ml) were added to the cells for 3 days, which were harvested later. DOC: sodium deoxycholate. B. Densitometry analysis: relative abundances were normalized to the top band of the control sample of the digitonin fraction. C. Quantitation of hydroxyproline in the pellets. (C: control; T: TGF-β1; A: AEEA; T + A: TGF-β1 + AEEA). These results were representative of at least three independent biological replicates.