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Overview of single vesicle analysis. (A) Overview of the procedural steps for single vesicle analysis. EV are biotinylated and captured on streptavidin-coated glass coverslips and stained by fluorescent antibodies. Coverslips are imaged by microscopy and multidimensional data are analyzed. Scale bar = 10 µm (B) Representative Nanosight Tracking Analysis (NTA) size distribution of vesicles from GBM8 media. NTA analyses are shown for samples before and after separation.
