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. Author manuscript; available in PMC: 2019 May 6.
Published in final edited form as: Sci Signal. 2017 Oct 10;10(500):eaal4055. doi: 10.1126/scisignal.aal4055

Fig. 3. TRPV1 inhibition rescues hyperthermia-induced congenital defects.

Fig. 3.

(A) Alcian blue and alizarin red stains of HH36 heads from hyperthermia-exposed and hyperthermia-exposed chicks following pretreatment with the TRPV1 inhibitor SB366791. Upper beak measurement extended from the quadratojugal (white arrowhead) to the tip of the upper beak (black arrowhead). (B) Comparison of upper beak length to femur length ratios in control, hyperthermia, or hyperthermia + SB366791 chicks. (C) Histological sections through the Aorta (Ao) and pulmonary trunk (P) comparing the luminal areas (arrows) of a control (top), hyperthermia (middle), and hyperthermia-exposed hearts with SB366791 pretreatment (lower) distal to the semilunar valves at the level of the left coronary artery (white arrowhead). Bar = 200μm. (D-E) Cavalieri probe estimates of the luminal cross-sectional area of the aorta (D) or pulmonary trunk (E) immediately above the aortic valve. Significance was determined by one-way ANOVA followed by Bonferroni’s multiple comparisons test. *P<0.05, **P<0.004, ***P<0.0001. The number of biological replicates is indicated by n in the graphs in (B), (D), and (E).