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. Author manuscript; available in PMC: 2020 Jun 1.
Published in final edited form as: Prostate. 2019 Mar 22;79(8):896–908. doi: 10.1002/pros.23796

FIGURE 2. Src kinase mediates high saturated fat diet accelerated prostate tumor progression.

FIGURE 2.

(A) PC-3 cells were transduced with shRNA-Control or shRNA-Src by lentiviral infection. The transduced cells were implanted into the flank sides of SCID mice subcutaneously (n = 9 per group). The mice were placed on a 10% low fat diet (LF), 40% saturated fat (HSF) diet, or 40% unsaturated fat (HUF) diet for 8 weeks. The dietary components including the amount and type of fatty acids are listed in Supplemental Table 3 and 4. All the diets contain 20% of total calories from proteins. The 10% LF diet contained 70% of the total calories from carbohydrate and 6% from saturated fat and 4% from unsaturated fat. Both the 40% HSF and HUF contain 40% of total calories from carbohydrate. While the 40% HSF has 36% of calories derived from saturated fat and 4% from unsaturated fat, the 40% HUF has 4% of calories from saturated fat and 36% from unsaturated fat. Representative images, sizes and weights of xenograft tumors are shown. (B) PC-3 cells were transduced with shRNA-control or shRNA-Src#1 or #2 by lentiviral infection. The expression levels of Src kinase were analyzed by immunoblotting. (C-D) Expression levels of Src kinase (C) and Ki-67 (D) in PC-3 xenograft tumors in panel A were examined by IHC staining (scale bars, 200 μm). *: p < 0.05; **: p < 0.01; N.S.: not significant.