Skip to main content
. Author manuscript; available in PMC: 2020 Jun 1.
Published in final edited form as: Prostate. 2019 Mar 22;79(8):896–908. doi: 10.1002/pros.23796

FIGURE 3. Biosynthesis of acyl-CoAs in prostate cancer cells and xenograft tumors.

FIGURE 3.

(A) The levels of palmitoyl-CoA in PC-3 xenograft tumors from mice on 10%, 45%, or 60% fat diet were analyzed by LC/MS-MS. The amount of palmitoyl-CoA was standardized to the amount of total protein in the tumors. Seven tumors per group were analyzed. (B) PC-3 or DU145 cells were grown in DMEM medium with 10% FBS, and treated with DMSO (control), decanoic acid (DA, C10:0), lauric acid (LA, C12:0), myristic acid (MA, C14:0), or palmitic acid (PA, C16:0) for 24 hours. (C-D) PNT2 or 22Rv1 cells were grown in RPMI1640 medium with 10% FBS and treated with DMSO or palmitic acid (PA, C16:0) for 24 hours. (E) 293T cells expressing doxycycline inducible Src(Y529F) were treated with decanoic acid (DA, C10:0), lauric acid (LA, C12:0), myristic acid (MA, C14:0), or palmitic acid (PA, C16:0) for 2 and 24 hours. Cellular palmitoyl-CoA was extracted as described in the Materials and Methods. Levels of cellular palmitoyl-CoA were analyzed by LC/MS-MS. The amount of cellular palmitoyl-CoA from different fatty acid treatments was divided with that in the DMSO control. Therefore, the ratio of palmitoyl-CoA in the DMSO treatment group was set as 1. (F) 293T cells expressing doxycycline inducible Src(Y529F) were treated with DMSO (Control) or palmitic acid (PA, C16:0) for 24, 48, 72 hours. The levels of palmitoyl-CoA were analyzed and compared with the levels in the DMSO control. The ratio of palmitoyl-CoA in the DMSO treatment group was set as 1 (three repeats in each group).