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. Author manuscript; available in PMC: 2020 May 1.
Published in final edited form as: J Struct Biol. 2019 Mar 14;206(2):193–203. doi: 10.1016/j.jsb.2019.03.003

Fig. 5. Binding assays of PAM-derived peptides to hPg at 15° C and 37° C.

Fig. 5.

hPg was immobilized on a CM-5 chip and peptide concentrations used for the titrations are indicated in each panel. Kinetic analyses were performed at 15° C for: (A) AGL55NS88.2, (B) KTI55SS1448, (C) VEK75AP53_RHI/AA. Steady state analyses were performed at 37° C for (D) AGL55NS88.2, (E) KTI55SS1448, (F) VEK75AP53_RH1/AA. In kinetic analyses, the experimental data are shown in black lines and fitted curves are shown in red lines. The corresponding binding constants from both kinetic and affinity analyses are provided in Table 6.