Table 4.

Weight-average molecular masses of naturally occurring PAMs^a

Proteinb	T (° C)	Molecular mass (Da)c	% Dimerd
PAMAP53	25	87,900 ± 1,000	100
	37	84,700 ± 4,500	97.5 ± 2.4
PAMNS88.2	25	81,900 ± 300	91.4 ± 3.3
	37	69,000 ± 800	55.5 ± 2.1
PAMNS223	25	83,700 ± 1,100	94.5 ± 2.8
	37	57,500 ± 2,500	33.7 ± 6.3
PAMNS455	25	83,400 ± 1,200	86.8 ± 3.3
	37	52,200 ± 2,600	20.4 ± 5.2
PAMSS1448	25	77,700 ± 400	86.7 ± 4.1
	37	69,800 ± 2,100	56.4 ± 2.5
PAMSS1572	25	83,200 ± 900	95.0 ± 5.0
	37	74,900 ± 2,000	75.3 ± 4.1
PAMSS1574	25	78,800 ± 1,200	100
	37	55,700 ± 2,600	33.5 ± 7.4

^aExperiments were not performed at 4° C since maximal dimeric forms were present at 25° C.

^bMolecular masses of the protein linear sequence from MALDI-TOF: PAM_AP53, 41,083 Da; PAM_NS88.2, 40,020 Da; PAM_NS223, 41,760 Da; PAM_NS455, 42,055 Da; PAM_SS1448, 38,644 Da; PAM_SS1572, 41,635 Da; PAM_SS1574, 37,401 Da.

^cWeight-average molecular mass of proteins in solution from AUC experiments at the specified temperatures. Data at 25° C were published previously (Qiu et al., 2018) and are listed here for comparison. Data from the top, middle, and bottom channels were collected and presented as mean ± S.D.

^dThe portion of observable dimeric species in solution was calculated using SEDPHAT. Data from the top, middle, and bottom channels were collected and presented as mean ± S.D.