Figure 4.

Thallium impacted the levels of 60S ribosomal subunits. (A) HEK293T was exposed to Tl(I) for 24 hours. The cells were lysed in 0.25 mM Mg²⁺ to dissociate the 40S and 60S ribosomal subunits. The resulting ribosomal profiles were analyzed by sucrose gradient sedimentation. (B) The resulting 60S:40S ratios were quantitated for control versus treatments as noted in the monosome profile analyses. The means from two biological repeats (n = 2) are displayed. Student’s t tests relative to the control were performed. **p < 0.01, ***p < 0.005, ****p < 0.001 (C) Ribosomal proteins of large subunit (i.e. RpL23, RpL36) and small subunit (i.e. RpS3a) were detected by immunoblotting. (D,E) NIH/3T3 (D) and CL1-0 (E) cells were treated with different amount of Tl(I) for 24 hours. Cells were harvested and lysed in the buffer with 0.25 mM Mg²⁺. Cell lysates were fractioned through 10% to 50% sucrose gradients and the profiles were recorded using A₂₅₄. Uncropped gel images are shown in Supplementary Fig. S4.