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. 2019 May 6;9:6958. doi: 10.1038/s41598-019-43490-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Dimer formation of Tr-ωTA. (a) Interactions between the two subunits of Tr-ωTA. Subunit A, B, and the interface are represented as orange, cyan, and grey spheres, respectively. (b) A cross section of subunit B containing PMP. PMP is depicted as sticks. (c) Loop insertion at the interface. The black dashed circles indicate inserted loops between the two subunits. The colour code is the same as in panel a. (d) SEC-MALS profile of Tr-ωTA. SEC-coupled MALS data (red) are plotted as elution volume and molecular mass distributions, and superimposed on the SEC chromatogram (black) at 280 nm.