Figure 3.
Inflation of the club at the beginning of the subadult stage. (a) Overview of the entire club. Confocal LSM scan. The bulb primordium occupies only a small portion within the club, the remaining club is inflated with haemolymph and this straightens out the previously wrinkled subadult cuticle. (b) Two different focal planes of CLSM scan showing details of bulb primordium morphology. Distal is to the left in all panels. Scale bar: 100 µm in all panels. Abbreviations: bu-pr, bulb primordium; cl, claw; cu, cuticle; hy, hypodermis.