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. 2019 May 6;9:6949. doi: 10.1038/s41598-019-42824-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phylogenetic neighbour-joining (NJ) tree of the vanD-positive E. faecium described in this report and the sequenced vanD-PCR fragments (~150 nt). NJ tree was generated and bootstrapped (1000 repetitions) from ClustalX2 multiple alignment of trimmed DNA sequences (Supplement A6). The type of vancomycin-resistance gene is denoted on the right-hand side of the figure, based on the most similar reported vanD gene. Further included isolates include the six Dutch vanD-positive isolates recently reported¹⁷ and reference genomes. Accession numbers are given in the figure. The tree was rooted using a randomly scrambled E. faecium fragment. Bar represents substitutions per site.