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. 2019 May 6;9:6941. doi: 10.1038/s41598-019-43497-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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TEM characterizes glial cells and axon ultrastructure within the larval zebrafish midgut. TEM reveals ultrastructure of myenteric plexus neuropil of the midgut in (A,B′) 7 dpf and (C,D′) 18 dpf larvae. Magenta-dashed box corresponds to region of magnification (A′–D′). Intestinal epithelium (IE), nucleus (N), muscularis (M), endothelial cell (EC), type 1 glial (T1G) and type 2 glia (T2G). Scale bars denote the following: (A) 500 nm, (A′) 250 nm, (B) 500 nm, (B′) 250 nm, (C) 500 nm, (C′) 250 nm, (D) 500 nm, (D′) 250 nm. Yellow arrows in (A′,B′) and white arrows in (C′) point to electron dense junctions between T1G and T2G.